Fig. 3
From: Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
SMAR1 makes a triple complex with PTBP1 and HDAC6. a Expression of hnRNP A1, hnRNP A2 and PTBP1 upon shRNA-mediated knockdown of SMAR1 in MCF7. b Co-IP of SMAR1 with hnRNP A1, hnRNP A2, PTBP1, and HDAC6 in MCF7 suggest that SMAR1 interacts with PTBP1 and HDAC6. c Co-IP of PTBP1 with SMAR1, HDAC1, and HDAC6 in MCF7 suggest that PTBP1 interacts with SMAR1 and HDAC6. d Co-IP of HADC6 with hnRNP A1, hnRNP A2, PTBP1, and SMAR1 in MCF7 confirms that HDAC6 interacts with PTBP1 and SMAR1. e Sequential Co-IP of SMAR1 with HDAC6 and PTBP1 in MCF7 suggest that SMAR1-HDAC6 makes a triple complex with PTBP1