Fig. 2
From: Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
SMAR1 regulates PKM alternative splicing. a qRT-PCR of PKM isoforms normalized to 18S rRNA and further normalized to constitutive PKM exon (exon 11) upon shRNA-mediated knockdown of SMAR1 in MCF7 (n = 3). b Expression of PKM isoforms upon shRNA-mediated knockdown of SMAR1 in MCF7 by western blot. c qRT-PCR of PKM isoforms normalized to 18S rRNA and further normalized to constitutive PKM exon (exon 11) upon Flag-SMAR1-mediated overexpression of SMAR1 in MDA-MB-231 (n = 3). d Expression of PKM isoforms upon Flag-SMAR1-mediated overexpression of SMAR1 in MDA-MB-231 by western blot. e Schematic representation of dual chromatic PKM minigene system. f Confocal microscopy to check the expression of eGFP and mCherry in MCF7 cells transfected with PKM minigene along with Flag-SMAR1. Relative fluorescence intensity of eGFP and mCherry was quantified with the help of ImageJ and fold change in eGFP/mCherry ratio was calculated for control and Flag-SMAR1 overexpression (n = 3). Error bars show mean values ± SD. Differences were considered statistically significant with *p < 0.05, **p < 0.01, and ***p < 0.001; ns non-significant difference (p > 0.05)