Skip to main content
Fig. 4 | Cancer & Metabolism

Fig. 4

From: GLUT5 (SLC2A5) enables fructose-mediated proliferation independent of ketohexokinase

Fig. 4

Fructose fluxes through HK, not KHK, in order to sustain cellular proliferation. a Percent of heavy isotope (13C) incorporation into fructose, fructose 1-phosphate (F1P), and lactate as detected by LC/MS from polar extracts of PC3, semi-trained PC3, and trained PC3 cells (n = 2-3). The isotopic labelling is indicated by M+# designation indicated in the legend where the # represents the amount of [12C] replaced by [13C]. Two-tailed unpaired t tests were used between parental and trained cells (M+3 for lactate, M+6 for fructose/F1P). *P < 0.05, **P< 0.01, and ****P < 0.0001. b Total abundance of fructose, F1P, and lactate as detected by LC/MS from polar extracts of PC3, semi-trained PC3, and trained PC3 cells (n = 2–3). Two-tailed unpaired t tests were used between parental and trained cells. *P < 0.05, **P< 0.01, and ****P < 0.0001. c Extracellular acidification rate (ECAR) over time of PC3, semi-trained PC3, and trained PC3 cells under basal conditions and following the addition of glucose, oligomycin (Oligo), and 2-deoxyglucose (2-DG) at the indicated times. Data are the mean and SEM from 6 replicates. d ECAR over time of PC3, semi-trained PC3, and trained PC3 cells under basal conditions and following the addition of fructose, Oligo, and 2-DG at the indicated times. Data are the mean and SEM from 6 replicates. e GLUT5 or an empty vector (EV) were overexpressed in 293T or 293T KHK-/- cells. The cells were plated at 20,000 cells/well and then grown in the presence of no sugar, 10 mM fructose, or 10 mM glucose. After 7 days, the cells were fixed, stained with crystal violet solution (n = 2 per condition). f Fold change in cell viability as assessed by ATP concentration (Cell Titer Glo) of the indicated fructolytic cell lines grown in either 10 mM glucose or 10 mM fructose containing the specified concentrations of 2-DG after 72 h (n = 3 per concentration). The half maximal inhibitory concentration (IC50) is displayed on the graph for each curve. g Fold change in cell viability as assessed by ATP concentration (Cell Titer Glo) of the trained PC3 grown in the specified sugar conditions containing the specified concentrations of 2-DG after 96 h. (n = 2 per concentration). The half maximal inhibitory concentration (IC50) is displayed on the graph for each curve

Back to article page