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Fig. 3 | Cancer & Metabolism

Fig. 3

From: GLUT5 (SLC2A5) enables fructose-mediated proliferation independent of ketohexokinase

Fig. 3

GLUT5 overexpression rescues cellular proliferation in fructose. a Normalized expression of genes that are differentially expressed (q = 0.4, >1.1 log2 fold change) between PC3 and semi-trained PC3 cells (passage 20) presented in heatmap form. b Relative expression of SLC2A5 transcript in semi-trained PC3 and trained PC3 cells as compared to the parental PC3 line. Two primer sets were used (n = 2 per condition). Two-way ANOVA with Fisher’s LDS test. *P < 0.05, ****P < 0.0001. c Immunoblot of the indicated proteins using lysates from PC3, semi-trained PC3, and trained PC3 cells. The murine liver and muscle used as controls. d GLUT5 or an empty vector (EV) were overexpressed in the indicated cells lines. The cells were plated at 20,000-30,000 cells/well and then grown in the presence of no sugar, 10 mM fructose, or 10 mM glucose. After 3 days, the cells were fixed and stained with crystal violet solution (n = 2 per condition)

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