Fig. 1

Cellular gene expression and tissue of origin do not determine cellular proliferation in fructose. a PC3 and HepG2 were seeded into 12-well plates (20,000 cells/well) and cultured in the absence or presence of 10 mM fructose, or 10 mM glucose media for approximately 3 days. Cell density (% confluency) was monitored over time using live cell imaging (n = 2 per media condition). b Fructolytic index (fructose-mediated growth/glucose-mediated growth) of the indicated cell lines arranged in order of least to most fructolytic (n = 3). c Fructolytic index of cell lines in b grouped by tissue of origin. d Normalized expression of the indicated genes for each cell line shown as a heatmap. Cell lines ordered by fructolytic index (n = 2 per gene per cell line). *Denotes C_t > 30. e Immunoblot of the indicated proteins using lysates from the indicated cell lines, ordered from least to most fructolytic. The murine muscle, liver, and Khk knockout liver were used as controls