Fig. 6

Role of transforming grow factor beta (TGF-β) in the relationship between PD-L1 and Chk-α. Relative fold change of TGF-β mRNA expression in MDA-MB-231, shCOX-2-MDA-MB-231, SUM-149, Pa09C, and Pa20C cells: untreated, transfected with 100 nM luciferase siRNA, transfected with 100 nM scrambled siRNA, transfected with 100 nM Chk-α siRNA, transfected with 100 nM PD-L1 siRNA #1, and transfected with a mixture of 50 nM PD-L1 and 50 nM Chk-α siRNA. Values presented as box and whisker plots, with the middle line representing the mean and the whiskers representing the maximum to minimum data points from 4 to 7 independent experiments. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, compared to untreated cells. ^#p ≤ 0.05, ^##p ≤ 0.01, ^###p ≤ 0.001, compared to cells transfected with luciferase siRNA. ⁺p ≤ 0.05, ⁺⁺p ≤ 0.01, ⁺⁺⁺p ≤ 0.001, compared to cells transfected with scrambled siRNA