Fig. 6

Involvement of β-catenin pathway in the biological functions of RNF115 in LUAD cells. a–d H1299 cells transfected with oeRNF115 or Vector control were treated by XAV939 or DMSO (vehicle). a Measurement of cell proliferation based on CCK-8. b Extracellular acidification rates for glycolysis. c Oxygen consumption rates for mitochondrial respiration. d The protein expressions of cytoplasmic HK-2 and LDHA with GAPDH as the loading control and β-catenin in nucleus with H3 as the loading control by western blotting. e–g H358 or H1975 cells transfected with shRNF115 (1# and 2#) or control shRNA (NC) and oeβ-catemom or Vector control. e Expression of β-catenin in nucleus by western blotting with the loading control as H3. f Extracellular acidification rates for glycolysis. g Oxygen consumption rates for mitochondrial respiration **P < 0.01