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Fig. 5 | Cancer & Metabolism

Fig. 5

From: RNF115 promotes lung adenocarcinoma through Wnt/β-catenin pathway activation by mediating APC ubiquitination

Fig. 5

Effects of RNF115 on β-catenin regulators and APC ubiquitination in LUAD cells. a Expressions of RNF115, Axin1, APC, and GSK3β proteins in H358 cells stably expressing shRNF115 (1# and 2#) or control shRNA (NC) by western blotting with the loading control as GAPDH. b The transcriptional levels of APC in H358 cells stably expressing shRNF115 (1# and 2#) or control shRNA (NC) by qRT-PCR. c H1299 cells transfected with oeRNF115 or Vector control were treated by MG132 or DMSO (vehicle). APC expression was detected by western blotting. d Association between RNF115 and APC by co-immunoprecipitation. e Immunoprecipitation and immunoblotting of APC in H358 cells stably expressing shRNF115 (1#) or control shRNA (NC)

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Cancer & Metabolism

ISSN: 2049-3002