Fig. 2From: RNF115 promotes lung adenocarcinoma through Wnt/β-catenin pathway activation by mediating APC ubiquitinationEffectiveness of RNF115 expression on proliferation and apoptosis in LUAD cells. a–e Comparison of RNF115 expression, cellular proliferation, and apoptosis between H358 or H1975 cells transfected with shRNF115 (1# and 2#) and control shRNA (NC). a Expression of RNF115 protein by western blotting with the loading control as GAPDH. b, c Measurement of cell proliferation by CCK-8 assay (b) and Click-iT EdU Cell Proliferation Assay (c). d The ratio of apoptotic cells. ***P < 0.001 vs NC. e Expression of PCNA and Cleaved caspase-3 by western blotting. f–i Comparison of RNF115 expression, cellular proliferation, and apoptosis between H1299 cells transfected with oeRNF115 and Vector control. f Expression of RNF115 protein by western blotting with the loading control as GAPDH. g, h Measurement of cell proliferation by CCK-8 assay (g) and Click-iT EdU Cell Proliferation Assay (h). i The ratio of apoptotic cells. **P < 0.01 and ***P < 0.001 vs VectorBack to article page