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Table 2 Stoichiometric constants and correction factors used for intact cell JATP estimation

From: Aglycemic growth enhances carbohydrate metabolism and induces sensitivity to menadione in cultured tumor-derived cells

Substrate (S)

Max O/mol S

Max H+ pumped/mol S

ATP from SCS

Total ATP (mol S−1)

P/O (total ATP/mol O)

d-Glucose - > CO2 (MAS active)

12

110

2

33.45

2.78

Pyruvate - > CO2

5

46

1

13.27

2.654

Glutamine - > CO2 (pyruvate cycle active)

8

62

2

18.9

2.36

  1. ATP/O ratios for substrate refeeding conditions were estimated as previously described [37]. The following values were assumed: 11/3 H+/ATP phosphorylated; 2e-/O; 2NADH (or 2QH2)/O2; 1ATP/GTP at succinyl CoA synthetase (SCS). Malate aspartate shuttle (MAS)
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Cancer & Metabolism

ISSN: 2049-3002