Fig. 1

Aglycemic growth adaptation did not alter mitochondrial content or network complexity. a Metabolic pathway representation of carbon fuel sources relevant to the growth conditions used in this study. b Graphical summary of the experimental design. c Representative laser scanning confocal images of HEPG2 cells stained with nuclear counterstain (Hoechst) and mitochondria-localized dye (TMRM). d Nuclear and mitochondrial volumes and the ratios of mitochondrial to nuclear volumes. N = 30 cells/group—3 biological replicates. e Log scale probability of mitochondrial cluster masses P(M). N = 30 cells/group (f) Cluster entropy (h). RandClust indicates simulated maximum entropy value, SingleClust indicates minimum possible entropy value. N = 30 cells/group—3 biological replicates. Data are mean ± SEM. Means were compared using Student’s t test. *p < 0.05 HEPG2-Glc vs. HEPG2-Gal. Scale bars in images are 25 μm