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Fig. 4 | Cancer & Metabolism

Fig. 4

From: SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice

Fig. 4

SIRT6 enhances OXPHOS and energy status in MCF7 cells. a Western blot analysis of PDH expression in MCF7 cells transduced with human WT or catalytically inactive (H133Y) SIRT6 or with a control vector (VECTOR). PDH expression was quantified following normalization to GAPDH. b-g Activity of mitochondrial complexes (b–d), oxygen consumption (e), activity of Fo-F1 ATP synthase (f), and energy status, expressed as ATP/AMP ratio (g), were measured in MCF7 cells transduced with human WT or catalytically inactive (H133Y) SIRT6 or with a control vector (VECTOR). Data are presented as mean ± SD of three different experiments. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not statistically significant. h Western blot analysis of PDH expression in MCF7 cells transduced with an shRNA targeting SIRT6 (SIRT6-sh) or with a control vector (VECTOR). PDH expression was quantified following normalization to GAPDH. i–n Activity of mitochondrial complexes (i–k), oxygen consumption (l), activity of Fo-F1 ATP synthase (m), and energy status, expressed as ATP/AMP ratio (n), were measured in MCF7 cells transduced with an shRNA targeting SIRT6 (SIRT6-sh) or with a control vector (VECTOR). Data are presented as mean ± SD of three different experiments. *P < 0.05, ***P < 0.001. o Protein lysates were generated from MCF7 that were engineered with a control vector (VECTOR) or with an shRNA targeting SIRT6 (SIRT6-sh)\. Phosphorylated (Thr183, Thr172) and total AMPK as well as GAPDH were detected by Western blot (one representative experiment out of three is presented)

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