Fig. 2
From: SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice
SIRT6 silencing reduces tumor growth in MDA-MB-231 xenografts. a, b 2 × 106 MDA-MB-231 BC cells transduced with either a SIRT6-shRNA or a control vector were injected subcutaneously in both flanks of BALB/c athymic nude mice (Hsd:Athymic Nude-Foxn1nu) (VECTOR, n = 8 and SIRT6-sh, n = 10). In a, mice were sacrificed when the tumors became palpable; tumors were used for protein lysate generation, and SIRT6 and α-tubulin were detected by Western blot. In b, tumor volume was monitored over time from the day of tumor cell inoculation. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (statistical values refer to the comparison between tumors with silenced SIRT6 and control tumors at the same time point). c Fifty days after tumor cell inoculation, mice were sacrificed, and masses were collected, imaged, and weighted (VECTOR: n = 6; SIRT6-sh: n = 6). Data are presented as mean ± SD. **P < 0.01