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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Pharmacological activation of pyruvate kinase M2 reprograms glycolysis leading to TXNIP depletion and AMPK activation in breast cancer cells

Fig. 3

DASA-58 affects respiration levels in BCa cells without an indication of mitochondrial damage. a, b Online measurement of oxygen saturation in culture medium using oxygen sensors embedded in 24-well plates in MCF7 (metformin and 2DG were used as controls) (a) and in four different BCa cell lines in response to either DASA-58 (30 μM) or TEPP-46 (30 μM) (b). c Mitochondrial membrane potential measured with JC-1 dye in different BCa cell lines in response to DASA-58 (15 μM) showing a slight increase or no change in membrane potential in all tested cell lines and up to 72 h of treatment (data presented as red/green fluorescence fold change in comparison to mock treatment). d Online oxygen consumption in isolated mouse liver mitochondria using oxygen sensors embedded in 96-well plate showing no direct effect of neither DASA-58 (30 μM) nor TEPP-46 (60 μM) on mitochondrial activity, 5 μM of rotenone (complex I inhibitor) and 1µg/mL of oligomycin (ATP synthesis inhibitor) are used as control

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