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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Intracellular arginine-dependent translation sensor reveals the dynamics of arginine starvation response and resistance in ASS1-negative cells

Fig. 5

Effects of intracellular arginine concentrations on translation. a Arginine concentrations in SKLMS1 WT and LTAT cells expressing GFP ArgSen over time with ADI-PEG20 treatment as measured by mass spectrometry. b Citrulline concentrations in SKLMS1 WT and LTAT cells expressing GFP ArgSen over time with ADI-PEG20 treatment. c Average GFP ArgSen fluorescence over 2 h with various concentrations of arginine in media in three cell lines. Horizontal dotted lines indicate the values with ADI-PEG20 treatment. Vertical dotted lines indicate calculated intracellular arginine concentrations after 48 h of ADI-PEG20 treatment. d Effective concentration (EC) values for arginine driving translation of GFP ArgSen in three cell lines, calculated from c. e Histograms of cell volumes after 48 h of ADI-PEG20 treatment. f Intracellular arginine concentrations after 48 h of ADI-PEG20 treatment, along with values used to calculate concentrations. All error bars represent standard deviation

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Cancer & Metabolism

ISSN: 2049-3002