Fig. 2

PFK15 reduces proliferation and induces death in MIA PaCa-2 but not human pancreatic stellate cells: PDAC cell lines:MIA PaCa-2, BxPC-3 and PANC1 were treated with 0.3–10 μM PFK15 for up to 96 h; cell proliferation was measured using a sulforhodamine-B assay (a-c). Western blot for PFKFB3 in MIA PaCa-2, BxPC-3 and human pancreatic stellate cells (HPSCs) (di). Western blot for PARP cleavage (dii), cells were treated with PFK15 (3–10 μM) or vehicle (DMSO) for 6 h and bands were quantified; the intensity of cleaved PARP is expressed as a percentage of total PARP (e). Points and bars represent the mean ± SEM of 5 separate experiments. Kruskall-Wallis test; p < 0.05*