Fig. 1

OS cells are sensitive to glutamine depletion. The proliferation responses of OS MG63.3 cells to the deprivation of three carbon source nutrients: glucose (a), glutamine (b), both glucose and glutamine (c), or pyruvate (d). e The growth inhibition of MG63.3 cells with the treatment of glutaminase inhibitors (Compound 968, CB-839 and BPTES) for 48 h is represented as percentage of DMSO-treated control. f The proliferation responses of OS MG63.3 cells to varies concentration of CB-839 treatment for 6 days. Statistic analysis used ANOVA followed by multiple comparisons “vehicle vs each treatment,” *p < 0.05, **p < 0.01, ***p < 0.001