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Fig. 4 | Cancer & Metabolism

Fig. 4

From: Estrogen-related receptor alpha directly binds to p53 and cooperatively controls colon cancer growth through the regulation of mitochondrial biogenesis and function

Fig. 4

Targeting ERRα affects mitochondrial biogenesis and function dependent on p53. a IB analysis was performed using anti-ERRα, anti-p53, and anti-actin. b IB analysis was conducted using the anti-total OxPhos WB antibody cocktail, anti-VDAC1, and anti-HSP-90. c, d Mitochondrial DNA copy number analysis and the intracellular ATP levels were measured as described in the “Methods” section. e Cells were stained with MitoTracker Red CMXRos and DAPI and analyzed by flow cytometry. The percentage of the mitochondrial mass (mitoHigh) was quantified. f IF analysis to detect COX-4 and VDAC1 was conducted. Enlarged panels represent selected digitally enlarged portions of parent images to enhance the visibility of COX-4 and VDAC1. Co-localization of COX-4 and VDAC1 was quantified (as % overlay). Scale bar, 50 μm. All experiments were conducted using HCT-116p53+/+ and HCT-116p53−/− cells treated for 48 h with XCT790 (15 μM) or vehicle (DMSO). Data are shown as means ± S.D. (n = 2–3). The p value was calculated using a two-tailed Student’s t test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; n.s., not significant

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