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Fig. 6 | Cancer & Metabolism

Fig. 6

From: Protein synthesis inhibitors stimulate MondoA transcriptional activity by driving an accumulation of glucose 6-phosphate

Fig. 6

The MondoA-dependent transcriptional response to translation inhibition. mRNA sequencing was used to determine gene expression changes in HeLa and HeLa:MondoA-KO cells following 4-h treatments with 100 nM RocA. a Heatmap depicting the top 500 genes regulated by RocA treatment. Regression analysis using DESeq2 was performed to generate a genotype:treatment interaction scores. b A volcano plot showing log2(fold-change) of HeLa cells treated with RocA compared to HeLa:MondoA-KO cells treated with RocA. Genes with an adjusted p value ≤ 1E−10 are indicated in blue (downregulated) and red (upregulated). c Overrepresentation analysis was used to determine pathways that are dysregulated in HeLa cells treated with RocA compared to HeLa:MondoA-KO cells treated with RocA. d Gene set enrichment analysis and leading-edge analysis was performed using all gene sets in the Molecular Signature Database (Broad Institute). HeLa cells treated with RocA were compared to HeLa:MondoA-KO cells treated with RocA. Nodes that contain at least 4 gene sets are shown

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