Fig. 1
TXNIP expression is suppressed by translation. a Heatmaps showing TXNIP mRNA relative to ribosomal protein L24 in the genotype-tissue expression project (GTEx) database. Spearman correlation statistics are reported. b, c TXNIP mRNA levels in HeLa cells following 16-h treatments with the translation elongation inhibitors CHX (50 μg/mL), emetine (50 μg/mL), and puromycin (100 μg/mL) or the translation initiation inhibitor Rocaglamide A (RocA, 100 nM). d Relative rate of 3H-2-deoxyglucose uptake in HeLa cells following 16-h treatments with RocA or vehicle. e, f TXNIP mRNA and protein levels of the indicated proteins in HeLa cells transfected with a pool of four siRNAs against EIF4E or a pool of four scrambled siRNA controls. g TXNIP mRNA levels in HeLa cells following 16-h treatments with CHX or Torin (250 nM). TXNIP mRNA levels following 16-h CHX treatments of h C2C12 mouse myoblasts, i L6 rat myoblasts, and j 293T embryonic kidney cells. TXNIP mRNA levels were determined using RT-qPCR