Fig. 4From: The conversion of formate into purines stimulates mTORC1 leading to CAD-dependent activation of pyrimidine synthesisIncorporation of 15N into pyrimidines. a Schematic representation of the pathway of incorporation of 15N from [15N-amide]-glutamine into pyrimidine precursors and pyrimidines. Dashed lines indicate multiple chemical transformations. b–f Peak areas of 15N pyrimidine precursors and pyrimidines in HAP1-ΔSHMT2 cells at different times and under different conditions. Quantifications are done relative to the Taurine peak area. Bars represent the mean and error bars the standard deviation from 3 independent experiments, each represented by the symbols. The p values shown were obtained using a t test with two tails and unequal varianceBack to article page