Fig. 4

Incorporation of ¹⁵N into pyrimidines. a Schematic representation of the pathway of incorporation of ¹⁵N from [¹⁵N-amide]-glutamine into pyrimidine precursors and pyrimidines. Dashed lines indicate multiple chemical transformations. b–f Peak areas of ¹⁵N pyrimidine precursors and pyrimidines in HAP1-ΔSHMT2 cells at different times and under different conditions. Quantifications are done relative to the Taurine peak area. Bars represent the mean and error bars the standard deviation from 3 independent experiments, each represented by the symbols. The p values shown were obtained using a t test with two tails and unequal variance