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Fig. 3 | Cancer & Metabolism

Fig. 3

From: The conversion of formate into purines stimulates mTORC1 leading to CAD-dependent activation of pyrimidine synthesis

Fig. 3

a Representative immunoblotting in HAP1-ΔSHMT2 cells under the indicated conditions. b Quantifications of CAD phosphorylation related to a. c Immunoblotting in MDA-MB-231-ΔSHMT2 cells under the same conditions. d Representative immunoblotting in HAP1-ΔSHMT2 cells under the indicated conditions. e Quantifications of CAD phosphorylation related to d. f Immunoblotting in HAP1-WT cells under the indicated conditions. Doses: 1 mM formate, 50 nM rapamycin, 50 μM L-alanosine, 1 μM SHIN1 and 16 μM thymidine + 100 μM hypoxanthine (HT). Bars represent the average of 3 independent experiments, error bars the standard deviation and symbols data from the individual experiments. The reported p values were calculated using a one-sample t test (Ctrl vs formate) or a two-samples t test with unequal variance and two-tailes (formate vs formate + rapamycin, formate vs formate + alanosine)

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Cancer & Metabolism

ISSN: 2049-3002