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Fig. 1 | Cancer & Metabolism

Fig. 1

From: Disruption of redox homeostasis for combinatorial drug efficacy in K-Ras tumors as revealed by metabolic connectivity profiling

Fig. 1

Metabolic phenotyping of human K-RasG12S A549 (A549) and K-RasG13D HCT116 (HCT116) cancer cell lines. a Proliferation curves of A549 and HCT116 cancer cells. Cells were plated onto 6-well plates in standard medium. The culture medium was replaced after 18 h with standard medium ( , Glc 25 mM), or medium containing 1 mM glucose ( ), or 0.5 mM glutamine ( ). Cells were collected and counted at the indicated time points. Error bars indicate SD (n = 3). b Extracellular uptake ( ) of Glc/Gln and secretion ( ) of Lac/Glu in lung and colon cancer cells grown for 48 h. c Untargeted metabolic profiling of A549 lung and HCT116 colorectal cancer cell lines grown in standard growth condition. Hierarchical clustering heatmaps show significantly different intracellular metabolites by LC-MS and GC-MS. d, e Circos plots show the most significant up-regulated metabolites and cognate enriched pathways in A549 cells (panel c d) and HCT116 (panel d e). (p ≤ 0.05)

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