Fig. 3

Resistant cells are sensitive to PPP inhibition. a Graph depicting clonogenic survival of resistant and sensitive cells treated with 6AN for 48 h at indicated concentrations (n = 4). b Clonogenic survival assay was performed on drug-sensitive and drug-resistant cells treated with erlotinib in combination with 6AN (48-h combination treatment) (n = 3). c Cell cycle analysis was performed on resistant cells treated with 6AN (48 h) by flow cytometry using propidium iodide stained cells (n = 4). Effect of 6AN on cyclins was ascertained using immunoblot analysis (n = 3). d Effect of G6PD knockdown (72-h post siRNA transfection) on MiaPaCa/Erlo cell cycle distribution and cyclin levels was determined (n = 2). e Effect of G6PD knockdown on MiaPaCa/Erlo cell sensitivity to erlotinib was determined using clonogenic assay. Comparative effect of G6PD siRNA on survival of MiaPaCa2 and MiaPaCa/Erlo cell was also determined in same experiment (n = 2). f MiaPaCa2 cells transfected with G6PD overexpression plasmid (G6PD/pRK5) were analyzed for their sensitivity to erlotinib using clonogenic survival assay (n = 3). The results were compared with empty vector transfected MiaPaCa/Erlo cells that were treated with erlotinib. Data presented as average ± SEM (*p < 0.05, ^#p < 0.01)