Fig. 6
From: Treatment of ErbB2 breast cancer by mitochondrial targeting
Rescue of MEDICA effects by NDI1. a ErbB members and Akt were measured in BT474 cells infected with empty or NDI1 virus and cultured in galactose. Cells were treated for 40 h with MEDICA as indicated. Representative blots. b mTORC1 downstream targets were measured in BT474 cells infected with empty or NDI1 virus. Cells were treated for 24 h with MEDICA as indicated. Representative blots. c Inhibition of ErbB signaling and mTORC1 by MEDICA is partly rescued by infected NDI1. Conditions as in a and b. Mean ± SD. *Significant as compared to respective control (P < 0.05). #Significant as compared to empty virus (P < 0.05). d BT474 cells infected with empty or NDI1 virus were cultured in galactose medium and treated for 72 h with MEDICA as indicated. Mean ± SD. *Significant as compared to empty virus (P < 0.05). e BT474 spheroids prepared from cells infected with empty or NDI1 virus were allowed to form for 4 days followed by treatment for 8 days with MEDICA or phenformin as indicated. *Significant as compared to respective control (P < 0.05). #Significant as compared to empty virus (P < 0.05)