Fig. 4
Characterization of ribosomal proteins expression. a Biplot showing the log2 RPKM of RPFs (y-axis) and mRNA (x-axis) of genes. The expression of ribosomal proteins is highlighted in bright colors. Heatmaps of the log2 TMM of mRNA (b) and RPFs (c) of ribosomal protein genes, respectively. The color code represents expression data levels: light shades of blue indicate low expression levels (low log2 TMM values) while strong shades indicate high expression levels (high log2 TMM values). Biplots showing the log2-fold RPKM differences in RPFs (y-axis) and mRNA (x-axis) in ribosomal protein expression between MKL1 ΔN200 and MCF7 control (d), MKL1 ΔC301 and MCF7 control (e), and MKL1 ΔN200 and MKL1 ΔC301 (f) respectively. g Polysome profiles of MCF7 control and MKL1 ΔN200. Polysomal fractionation was done as described [15] with some modifications. Relative amount of RNA values refers to the inverse of the corrected Cq values (corrected Cq−1) from qRT-PCRs experiments. Error bars represent standard deviation from experimental triplicate measurements. RPKM, reads per kilobase million; TMM, trimmed mean of M values