Fig. 2

Differential gene expression at transcriptional and translational levels reveal a basal-like gene expression signature in MCF7 MKL1 ΔN200 cells. Heatmaps showing log2 TMM fold changes of genes with differential mRNA counts (a) and differential RPFs counts (b) genes between the cell lines. The color code represents fold changes levels: shades of red indicate a diminished expression while shades of blue indicate overexpression. c Gene set enrichment analysis (GSEA) showing the association of differentially expressed genes to luminal and d basal marker gene sets. The bar-code plot indicates the position of the genes on the expression data rank-sorted, with red and blue colors indicating over- and underexpression in MCF7 MKL1 ΔN200 compared to MCF7 MKL1 ΔC301 control cells, respectively. The enrichment plot for basal genes is skewed to the left, indicating an association of MCF7 MKL1 ΔN200 to the basal markers gene set. The enrichment plot for luminal genes is skewed to the right, indicating an association of MCF7 MKL1 ΔC301 control cells to the luminal marker gene set. Significance statistics for GSEA is shown on top of the gene set enrichment plot. TMM, trimmed mean of M values