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Fig. 6 | Cancer & Metabolism

Fig. 6

From: Fumarate hydratase-deficient renal cell carcinoma cells respond to asparagine by activation of the unfolded protein response and stimulation of the hexosamine biosynthetic pathway

Fig. 6

a RT-PCR results for GFPT2 gene. The first two panels of bars at each diagram represent the amino acids treatment resulted in expression fold change compared to untreated control in UOK262 (KO) and UOK262WT respectively. The dotted bar on the right side of each diagram represents the fold change of gene expression in UOK262 vs. UOK262WT cells treated with both amino acids (Asn + Gln). b Confirmative Western blot analysis of GFPT2 and Gln synthase proteins. The UOK262/UOK262WT cells treated with either Asn, Gln, or both for 96 h before cell homogenization. Twenty microgram of total protein loaded at each well unless stated otherwise. Actin used as a loading control. c Cytotoxicity effect of a pyrimidine inhibitor, teriflunomide, on UOK262/UOK262WT cell lines. The curves represent the percentage of untreated control and calculated after 96 h of incubation of the cells in different concentration of teriflunomide in the presence of either Asn, Gln, or both

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