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Fig. 3 | Cancer & Metabolism

Fig. 3

From: PHGDH supports liver ceramide synthesis and sustains lipid homeostasis

Fig. 3

Systemic PHGDH depletion is non-toxic. a Overall survival of mice expressing shPHGDH (N = 23) or the control shRenilla (shREN, N = 26). b Weight of male and female mice expressing shPHGDH (male, N = 16; female, N = 15) or shREN (male, N = 13; female, N = 10). Mice were placed on doxycycline at weaning. c Representative hematoxylin and eosin stained (N = 10+) and Ki-67 immunostained (N = 5 each) large intestine sections from shPHGDH and shREN mice at endpoint. d Representative hematoxylin and eosin stained bone marrow sections from shPHGDH and shREN mice (N = 10+) at endpoint. e Red blood cell counts of shREN (N = 31) and shPHGDH (N = 33) mice at 8 months. f White blood cell counts of shREN (N = 31) and shPHGDH (N = 33) mice at 8 months. g Oral glucose tolerance test at 100 days on doxycycline. Male and female shPHGDH and shREN mice were challenged with 2 g/kg glucose at time = 0, and blood glucose levels were assayed at the indicated time points. Male shPHGDH (N = 7), male shREN (N = 7), female shPHGDH (N = 6), female shREN (N = 10)

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