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Fig. 2 | Cancer & Metabolism

Fig. 2

From: PHGDH supports liver ceramide synthesis and sustains lipid homeostasis

Fig. 2

PHGDH knockdown impairs serine synthesis in vivo. a Fraction serum glucose labeling of shREN and shPHGDH mice infused with U-13C-glucose for 2.5 h. [13C]-label is denoted by the increase in mass (M) from M + 0 to M + n, where n denotes the number of labelled carbons. In addition to the expected 6 labeled carbons (M + 6), other isotopologues indicative of gluconeogesis were observed. bd Fraction glucose labeling of shREN and shPHGDH mouse pancreas (b), brain (c), and liver (d). Data represent the average and SD (n = 4 tissue samples from the 2 mice from [A] for each group). eg Fraction serine labeling of shREN and shPHGDH mouse pancreas (e), brain (f), and liver (g). Data represent the average and SD (n = 4 tissue samples from the 2 mice from [A] for each group). Total fraction labeling was compared with the Student t test. NS not significant. In addition to the expected 3 labeled carbons (M + 3), M + 1 labeling occurs as a consequence of folate cycle activity

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