Fig. 1
From: PHGDH supports liver ceramide synthesis and sustains lipid homeostasis
Generation of an inducible model for systemic PHGDH knockdown. a Schematic representation of the inducible shRNA system. b–e Western blot analysis of PHGDH, GFP, and HSP90 protein levels in the liver (b), pancreas (c), large intestine (d), and brain (e) of shPHGDH and shREN mice. ns, non-specific band. Mice were placed on a 200-ppm doxycycline diet for 4–8 months. f Serum serine concentrations of 8-month-old shREN (N = 16) and shPHGDH (N = 16) mice