Fig. 4

PHGDH and NPL dual KO does not impact growth rate and pyruvate generation from glucose. a Average proliferation rates of 3 PHGDH/NPL dual knockouts (dual KO) clones or wild-type PDACs with vehicle (−Dox) or PKM1/2 knockdown (+Dox). Cell counts were measured daily (n = 3). b ¹³C-Glucose labeling of intracellular metabolites for 60 min in wild-type (WT) or PHGDH/NPL dual knockout clone D7 cells with PKM1/2 knockdown for 60 min. The y-axis for all graphs is the percent labeling of indicated ¹³C-isotopologue. Experiments were performed in triplicates, and all data are displayed as the mean values ± standard error