Fig. 3

PHGDH knockout depletes intracellular serine, slightly decreases pyruvate generation, but does not affect cell proliferation. a Proliferation rates of wild-type A13M13 (PHGDH WT) or PHGDH knockout (PHGDH KO) populations of PDACs with vehicle (−Dox) or PKM1/2 knockdown (+Dox). Cell counts were measured daily (n = 3) after vehicle or doxycycline treatment. Western blot confirms PHGDH knockout in PDACs. b Relative intracellular metabolites levels are represented by peak intensities and are displayed relative to WT metabolite intensity averages. Cells are collected after 7 days of doxycycline treatment. Values are the average of three biological replicates. Error bars represent standard deviation. c ¹³C-Glucose labeling of intracellular metabolites for 24 h in WT and PHGDH KO PDAC clone P24 cells with PKM1/2 knockdown. The y-axis for all graphs is the percent labeling of indicated full labeled ¹³C-isotopologue. Experiments were performed in triplicates and all data are displayed as the mean values ± standard error. Statistically significant differences (p value) are marked with asterisks (*p < 0.05, **p < 0.01)