Skip to main content
Fig. 2 | Cancer & Metabolism

Fig. 2

From: Cysteine catabolism and the serine biosynthesis pathway support pyruvate production during pyruvate kinase knockdown in pancreatic cancer cells

Fig. 2

Pancreatic cancer cells upregulate the serine biosynthesis pathway following PKM1/2 knockdown. a Pool sizes of intracellular metabolites in PDAC cells treated with (+Dox) or without (−Dox) PKM1/2 knockdown were detected using ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). b 13C-Glucose flux to serine is upregulated in A13M13 PDAC cells with PKM1/2 knockdown (+Dox). Statistically significant differences (p value < 0.01) are marked with asterisks (**). c Metabolic pathways that may generate pyruvate from upstream glycolytic intermediates during PKM knockdown. NPL, N-acetylneuraminate pyruvate lyase; SDH, serine dehydratase

Back to article page