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Fig. 1 | Cancer & Metabolism

Fig. 1

From: Cysteine catabolism and the serine biosynthesis pathway support pyruvate production during pyruvate kinase knockdown in pancreatic cancer cells

Fig. 1

PKM2 and PKM1/2 knockdown do not decrease proliferation of pancreatic cancer cells. a, b PDACs proliferate at the same rate with (+Dox) or without (−Dox) PKM1/2 knockdown. Proliferation was assessed by counting cell numbers in triplicates for 4 days following 7 days of vehicle (−Dox) or 1 μg/ml doxycycline (+Dox) treatment to maximize PKM2 or PKM1/2 knockdown. Western blot confirms PKM1/2 knockdown in PDACs after 7 days of vehicle (−Dox) or doxycycline (+Dox) treatment. c Relative intracellular metabolites levels are represented by peak intensities and are displayed relative to −Dox averages. Values are the average of three biological replicates. Error bars represent standard deviation. Statistically significant differences (p value < 0.05) are marked with asterisks (*). d 13C-glucose labeling of intracellular metabolites for 24 h in A13M13 PDAC cells with vehicle (−Dox) or PKM1/2 knockdown (+Dox). The y-axis for all graphs is the percent labeling of indicated 13C-isotopologue

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