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Fig. 2 | Cancer & Metabolism

Fig. 2

From: Tissue of origin dictates GOT1 dependence and confers synthetic lethality to radiotherapy

Fig. 2

Metabolic profiles of PDA and CRC. a Relative metabolite levels as determined by LC/MS for glycolysis, pentose phosphate pathway (PPP), and serine metabolism in parental PDA (red) and CRC (blue) cell lines. Uniformly labeled (M+3, hashed bars) and unlabeled (M+0, solid bars) metabolite pools derived from U-13C-glucose (Glc, left) or U-13C-glutamine (Gln, right) for b lactate (Lac) and c alanine (Ala) as determined by LC/MS. d Relative U-13C-Glc-labeling of serine (Ser) and e glycine (Gly), as determined by gas chromatography (GC)/MS. f Ion currents for isotopologue distribution of citrate (Cit) derived from U-13C-Glc (left) or U-13C-Gln (right) in PDA and CRC cell lines. g Schematic summary of metabolic patterns observed in parental PDA and CRC cells. Red represents increased pool sizes in GOT1-sensitive PDA cells and blue represents increased metabolite pools in GOT1-insensitive CRC cells. 3-pSer, 3-phosphoserine; 3PG, 3-phosphogycerate; 6PG, 6-phosphogluconate; Ac-CoA, acetyl-CoA; αKG, alpha-ketoglutarate; B(1,3)PG, 1,3-bisphosphoglycerate; B(2,3)PG, 2,3-bisphosphoglycerate; Cit, citrate; DHAP, dihydroxyacetone phosphate; E4P, erythrose 4-phosphate; F6P, fructose 6-phosphate; FBP, fructose-1,6-bisphosphate; Fum, fumarate; G3P, glycerol-3-phosphate; G6P, glucose 6-phosphate; GA3P, glyceraldehyde 3-phosphate; GdL6P, glucono-delta-lactone 6-phosphate; Iso, isocitrate; Mal, malate; OAA, oxaloacetate; PEP, phosphoenolpyruvate; PHP, phosphohydroxypyruvate; PRPP, phosphoribosyl pyrophosphate; Pyr, pyruvate; R5P, ribose 5-phosphate; S7P, sedoheptulose-7 phosphate; SBP, sedoheptulose-1,7-bisphosphate; Suc, succinate. Error bars represent s.d. from biological replicates (n = 3). Stacked P values are presented for isotopologues in be and correspond by color. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; Student t test (unpaired, two-tailed)

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