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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate

Fig. 5

EGCG increases radiosensitivity in HCT116-IDH1wt/R132H cells. a Colony formation assay of HCT116-IDH1wt/R132H cells shows increased sensitivity to IR after treatment with EGCG compared to in HCT116. Surviving fractions were 5% (± 0.5%) vs. 16% (± 2%) for IDH1wt/R132H and IDH1wt cells, respectively, when cultured with 100 μM EGCG and irradiated with 4Gy. b Western blot quantification of phosphorylated H2AX foci in HCT116-IDH1wt/R132H and HCT116 cells, cultured with or without 100 μM EGCG and irradiated at 2 or 4 Gy. Endogenous H2AX-phosphorylation was higher in HCT116-IDH1wt/R132H cells than in HCT116 cells. Thirty minutes after IR, γH2AX levels further increased to higher levels in HCT116-IDH1wt/R132H than in HCT116 cells. The same trend could be observed in HT1080 (+ AGI-5198) cells (c)

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