Fig. 4
LC-MS analysis of D-2-HG pools after treatment with EGCG. HCT116-IDH1wt/R132H cells were incubated in DMEM with 10% FCS, supplemented with glucose, glutamine, and/or glutamate as indicated in the presence or absence of EGCG (shaded vs. non-shaded bars respectively). a Total pools of D-2-HG after incubation in medium containing Glc and Gln (I), Glc and Glu (II), or Glc, Gln, and Glu (III). Total D-2-HG levels decreased when cultured with EGCG. Fractional enrichments (FE) of D-2-HG are displayed in b. Blue bars display the fractions of D-2-HG that were derived from Gln* (dark blue) and both Gln* and Glc* (light blue) after incubation in DMEM with Glc + Gln* (formulation Ia) and Glc* + Gln* (formulation Ib) respectively. Red bars display the fractions of D-2-HG that were derived from Glu* (dark red) and both Glu* and Glc* (light red) after incubation in DMEM with Glc + Glu* (formulation IIa) and Glc* + Glu* (formulation IIb) respectively, and the fraction of D-2-HG derived from Glu* (dark red) after incubation in DMEM with Glc, Gln, and Glu* (formulation III)