Fig. 2

Proliferation assays of the isogenic cell line pair HCT116 and HCT116-IDH1^wt/R132H with EGCG. a Doubling times of cells as measured on the xCELLigence. Doubling times for HCT116-IDH1^wt/R132H (red bars) were significantly higher compared to its wild-type counterpart (blue bars). Supplementation of EGCG to the culture medium increased doubling times in both cell lines, but this effect was more significant in HCT116-IDH1^wt/R132H. b SRB assay of HCT116 cells cultured with EGCG (dotted lines) or vehicle (solid lines). HCT116-IDH1^wt/R132H (red lines) cells had slower proliferation rates as compared to HCT116 (blue lines). Addition of AGI-5198 (gray lines) rescued the proliferation rates of IDH1^R132H cells to HCT116 baseline levels. EGCG inhibited proliferation of HCT116-IDH1^wt/R132H more than HCT116, and this inhibitory effect was abolished by AGI-5198. Similar results were obtained for HT10180 cells (c)