Fig. 6From: Epithelial to mesenchymal transition (EMT) is associated with attenuation of succinate dehydrogenase (SDH) in breast cancer through reduced expression of SDHCMitochondrial mass and morphology. Mitochondrial mass and morphology were compared in MCF10A (parental, epithelial) and MFC10A/TWIST (mesenchymal) cells. a–g Confocal microscopy and quantitative image analysis of immune-stained mitochondria (TOM20 + ATPB). a Based on confocal z-stacks, 3D-models of mitochondrial volume and filament structure were generated, as indicated from left to right in the two image panels. b Mean number of mitochondria per cell (Nm). c Mean mitochondrial total volume per cell (Vm,cell). d Mean mitochondrial total tubule length per cell (Lm,cell). e Size (volume) frequency distribution comparing mitochondria in MCF10A (parental) versus MCF10A/TWIST cells. f Volume fraction analysis of mitochondrial subclasses (size). g Surface area (S.A.) to volume (Vm) regression analysis of individual mitochondria in MCF10A (parental) and MCF10A/TWIST cells. The analysis comprised (parental/TWIST) 937/3643 mitochondria with total volume 9020/9554 μm3, in 30/61 cells (n). h–n Effects of TWIST overexpression on mtDNA and gene expression of mitochondrial proteins. h Amount of mtDNA in MCF10A/TWIST relative to parental MCF10A. i Protein expression (WB) of TOM20. j Protein expression of PGC1α, including MCF10A/SNAI2 cells. k mRNA expression of CPT1 and CYCS. l Protein expression (WB) of Drp1 (DMN1L) and Opa1. m mRNA expression of DMNL1 (Drp1), OPA1, MFN1, and MFN2. n mRNA expression of PINK1 and PARK2. Student’s t test was used for statistical analysis. Data are shown as mean ± SD. *p < 0.01Back to article page