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Fig. 6 | Cancer & Metabolism

Fig. 6

From: Epithelial to mesenchymal transition (EMT) is associated with attenuation of succinate dehydrogenase (SDH) in breast cancer through reduced expression of SDHC

Fig. 6

Mitochondrial mass and morphology. Mitochondrial mass and morphology were compared in MCF10A (parental, epithelial) and MFC10A/TWIST (mesenchymal) cells. ag Confocal microscopy and quantitative image analysis of immune-stained mitochondria (TOM20 + ATPB). a Based on confocal z-stacks, 3D-models of mitochondrial volume and filament structure were generated, as indicated from left to right in the two image panels. b Mean number of mitochondria per cell (Nm). c Mean mitochondrial total volume per cell (Vm,cell). d Mean mitochondrial total tubule length per cell (Lm,cell). e Size (volume) frequency distribution comparing mitochondria in MCF10A (parental) versus MCF10A/TWIST cells. f Volume fraction analysis of mitochondrial subclasses (size). g Surface area (S.A.) to volume (Vm) regression analysis of individual mitochondria in MCF10A (parental) and MCF10A/TWIST cells. The analysis comprised (parental/TWIST) 937/3643 mitochondria with total volume 9020/9554 μm3, in 30/61 cells (n). hn Effects of TWIST overexpression on mtDNA and gene expression of mitochondrial proteins. h Amount of mtDNA in MCF10A/TWIST relative to parental MCF10A. i Protein expression (WB) of TOM20. j Protein expression of PGC1α, including MCF10A/SNAI2 cells. k mRNA expression of CPT1 and CYCS. l Protein expression (WB) of Drp1 (DMN1L) and Opa1. m mRNA expression of DMNL1 (Drp1), OPA1, MFN1, and MFN2. n mRNA expression of PINK1 and PARK2. Student’s t test was used for statistical analysis. Data are shown as mean ± SD. *p < 0.01

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