Characterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an increased expression of lactate dehydrogenase B

Table 2 Ingenuity canonical pathways associated with the proteins identified as differentially expressed between MCF7Ecadvar and MCF7pcDNA3 cells. Results obtained with the IPA tool

Ingenuity canonical pathways	-log(p value)	Proteins involved
Glycolysis	0.0836	G6PI, TPIS, PGAM1, F16P1, ALDOA
Gluconeogenesis	0.0827	G6PI, PGAM1, F16P1, ALDOA, MAOX
Protein ubiquitination pathway	0.0535	PSB3, PSA6, HS90B, GRP75, HS71A, HS71B, HS90A, UBA1
14–3-3-mediated signaling	0.0486	1433 T, GRB2, PDIA3, 1433Z, VIME
p70S6K signaling	0.0483	1433 T, GRB2, PDIA3, EF2, 1433Z
PI3K/AKT signaling	0.0478	1433 T, HS90B, GRB2, 1433Z, HS90A
Pentose phosphate pathway (oxidative branch)	0.0439	6PGL, G6PD
Aldosterone signaling in epithelial cells	0.0431	HS90B, PDIA3, GRP75, HS71A, HS71B, HS90A
PPARα/RXRα activation	0.0413	HS90B, GRB2, GPDM, PDIA3, HS90A
Glucocorticoid receptor signaling	0.0410	HS90B, GRB2, ACTB, GRP75, HS71A, HS71B, HS90A

List of the ten canonical pathways most altered in MCF7Ecadvar cells compared with MCF7pcDNA3. They were ordered in a descending form according to the number of the negative of the logarithm of the p value assigned to each of them. The symbol of the proteins identified with differential expression levels between both cell lines that were involved in each pathway are also indicated in the table. PPARα peroxisome proliferator-activated receiver α, RXRα retinoid X receiver α

ISSN: 2049-3002