Fig. 2

CHCHD4 promotes basal and adaptive metabolic responses, and provides a proliferative advantage to tumour cells. a Graph shows intracellular l-lactate levels from control U2OS cells, CHCHD4 (WT)-expressing cells (WT.cl1, WT.cl3) and CHCHD4 (C66A/C68A)-expressing cells, expressed as the percentage of control cell levels, measured using a cellular lactate assay. n = 3. mean ± SD, n.s. not significant, **p < 0.01. b Graph shows ECAR (mpH/min) in cells described in a, incubated in glucose-free media and measured using a seahorse respirometer. Compounds were added as indicated to following final concentrations: glucose, 12.5 mM; oligomycin A, 1 μM; 2-deoxy-d-glucose, 50 mM. n = 3, mean ± SD. c Chart shows intracellular fluorescence of cells described in a incubated with PBS (unstained) or 2-NBDG for 20 min. n = 3, mean ± SD, **p < 0.01. d Graph shows relative growth of cells described in a, incubated over 96 h. Data are presented for each time point relative to 0 h. n = 3, mean ± SD, **p < 0.01