Fig. 4

PKM2 deletion does not alter PDAC tumor cell proliferation. a Tumor tissue sections from end-stage KP^−/−C Pkm2^+/+ and KP^−/−C Pkm2^flox/flox mice were stained with an antibody against Ki67 as shown. Scale bars represent 50 μm for all images at × 20 magnification (top) and 20 μm for all images at × 40 magnification (bottom). b Fraction of nuclei positive for Ki67 was assessed as shown. No significant difference in Ki67 positivity was observed in tumors arising in KP^−/−C Pkm2^+/+ and KP^−/−C Pkm2^flox/flox mice. c Tumor tissue sections from KP^−/−C Pkm2^+/+ and KP^−/−C Pkm2^flox/flox mice were stained with isoform-specific antibodies against PKM1 and against PCNA as shown. Scale bars represent 50 μm for all images at × 20 magnification (top) and 20 μm for all images at × 40 magnification (bottom). d Fraction of nuclei positive for PCNA was assessed as shown. No significant difference in PCNA positivity was observed in tumors arising in KP^−/−C Pkm2^+/+ and KP^−/−C Pkm2^flox/flox mice