Fig. 5

CD38 regulates extracellular NAD⁺ levels in mouse tissues. a Flow cytometry histogram plots gated on EpCAM+ epithelial cells from wild-type (CD38+/+) or knockout (CD38−/−) prostate and seminal vesicle cells and stained for surface expression of CD38. b, c NAD⁺ and NADH levels were measured using the NAD⁺/NADH-Glo assay normalized to total DNA in each tissue and presented relative to wild-type. Mean ± SEM of four replicates is shown. d, e NAD⁺ levels in intact seminal vesicle tissue (d) or in tissue after having removed the fluid (e) normalized to protein and presented relative to wild-type. Mean ± SEM of 2–5 replicates is shown. f NAD⁺ levels remaining in the media were measured 60 min after the addition of 800 nM exogenous NAD⁺ to purified seminal vesicle cells. Fold reduction in initial NAD⁺ levels, relative to wild-type, is shown. Mean ± SEM of duplicates is shown. g Relative intracellular NAD⁺ levels from wild-type seminal vesicle cells measured 60 min after the addition of 0 or 800 nM NAD⁺. Mean ± SEM of three replicates is shown