Fig. 4From: Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translationMTHFD2 is a short-lived and dynamically regulated protein. a Half-life of enzymes of the one-carbon pathway in in HeLa, C2C12, and NIH3T3 cell lines. b Cumulative frequency of all quantified enzymes (596, 576, and 605 respectively) half-life in three cell lines. Dotted lines indicate relative half-life of MTHFD2 in each cell line. c Effect of treatment of HCT-116 cells for 24Â h with 25Â mg/ml protein translation inhibitor cyclohexamide on MTHFD2 protein levels. d qRT-PCR measurement of effect of 48Â h serum starvation on mRNA of folate one-carbon enzymes. e Immunoblots of MTHFD1, MTHFD1L, MTHFD2, and beta-tubulin in HCT-116 grown in normal media or serum starved for 24 or 48Â h. f Immunoblot showing time course of MTHFD2 protein response to serum replenishment in HCT-116 cells. SS, serum-starved; rep, serum replenishment (time point indicated). g Quantification of MTHFD2 mRNA in HCT-116 cells in control media, serum starved (SS) for 48Â h, and serum starved followed by 24Â h serum replenishment (rep). h Levels of MTHFD2 protein in nuclear and cytosolic compartments in HCT-116 cells that were serum starved for 48Â h or in replenished media for 24Â h after serum starvation. The COX IV and Lamin were used as cytosolic and nuclear markers respectively. Numbers in f and g indicate fold changes relative to controlBack to article page