Fig. 9

Transcriptomic and metabolomic analysis reveals essential differences in metabolism of leukemia and lymphocytes. Sorted splenic CD8+ T cells from WT mice 8 days after LCMV infection, compared to sorted T-ALL cells from WT mice 6 weeks after adoptive transfer. RNA isolated from sorted cells and analyzed by RNA-seq a Volcano plot of gene expression differences. Differentially regulated genes include those with greater than 2 fold change in expression and multiplicity adjusted p value < 0.01. Blue dots are overexpressed in CD8+ T cells, red in T-ALL. b Hallmark pathways enriched in leukemia (positive values) and T cells (negative values) and their normalized enrichment score. Statistical significance shown by * (FWER p < 0.05). c Expression of differentially regulated metabolic genes from the KEGG database. d Expression of select metabolic genes by RT-PCR relative to β-actin and Rpl-19. Biological replicates, n = 3–4 mice, as indicated by individual data points