Fig. 7

HK2 is not required for Treg function in vivo. Mice with YFP+ HK2-deficient Treg cells were generated (FP3-Hk2^−/− mice). a CD4+ cells from spleens of adult WT and FP3-Hk2^−/− mice were stimulated under Treg promoting conditions and RNA was isolated from sorted YFP+ cells. Hk2 expression relative to β-actin (n = 3 WT, 4 FP3-Hk2^−/−). b–g WT and FP3-Hk2^−/− mice were aged to 6 months. b Representative H&E stained sections of indicated tissues, n = 4 mice. Scale bars show 100 um. c Mouse weight; biological replicates, n = 4 mice. d Titer of indicated auto-antibodies in serum; biological replicates, n = 4 WT, 7 FP3-Hk2^−/− mice. e Spleen weight; biological replicates, n = 5 WT, 6 FP3-Hk2^−/− mice. f Number of splenic Treg cells and g percent of viable CD4+ or CD8+ T cells that are CD62L^hiCD44^lo (naive) or CD44^hi (memory); biological replicates, n = 4 WT, 6 FP3-Hk2^−/− mice. In all panels unless otherwise noted, error bars represent mean ± SEM, experiments performed twice independently, single-tailed Student’s t test or two-way ANOVA with Sidak multiple comparisons correction, *p < 0.05; **p < 0.01. NS = not significant