Fig. 3

HK2 is dispensable for T cell differentiation. a–e Naïve CD4+ T cells were enriched from splenocytes isolated from adult WT and T-Hk2^−/− mice. Enriched naïve CD4+ T cells were stimulated with irradiated splenocytes under Th0, Th1, Th2, Th17, and Treg promoting conditions for 5 days. Percent of CD4+ T cells positive for viability or viable and positive for indicated transcription factor shown. Biological replicates, n = 4–8 (as indicated by individual data points) from at least two independent experiments. f–h Splenocytes were isolated from 8 to 12 week old WT and T-Hk2^−/− mice to analyze steady state levels of various populations. f Percent of viable CD4+ or CD8+ cells that are CD62L^hiCD44^lo (naive) or CD44^hi (memory). Biological replicates, n = 5 mice. g–h Percent of viable CD4+ T cells positive for the indicated transcription factor (g) or cytokine (h). Biological replicates, n = 5 mice. In all panels unless otherwise noted, error bars represent mean ± SEM, two-way ANOVA with Sidak multiple comparisons, *p < 0.05; **p < 0.01. NS = not significant