Fig. 2

HK2 is not required for T cell activation and proliferation. Naïve CD4+ and CD8+ T cells were enriched from splenocytes isolated from adult WT and T-Hk2^−/− mice. T cells were activated in vitro with anti-CD3/28-coated beads for 24–72 h. a Percent of cells positive for indicated activation markers by flow cytometry gated on CD4+ (left) or CD8+ (right) cells after 24 h stimulation. Biological replicates, n = 4 from at least two independent experiments. b CFSE dilution in activated CD4+ and CD8+ T cells after 72 h of stimulation. Representative image of n = 4 biological replicates from at least two independent experiments. Quantified by proliferation index on right, n = 3–4 biological replicates as indicated by individual data points. In all panels unless otherwise noted, error bars represent mean ± SEM, one-way or two-way ANOVA with Sidak multiple comparisons, *p < 0.05; **p < 0.01. NS = not significant