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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Inflammation-induced DNA methylation of DNA polymerase gamma alters the metabolic profile of colon tumors

Fig. 5

Reduced Polg expression increases glucose uptake and resistance to oxidative stress. a Polg gene expression in nontarget (NT) or Polg knockdown (KD) mock tumoroids by qPCR relative to NT organoids. Mean ± SEM. The data is from three independent experiments each with two biological replicates. *P < 0.05. b DNA from tumoroids as in a was used in qPCR assays with primer sets specific for two different regions of mitochondrial DNA or the genomic ApoB. Mean ± SEM. The data is from three independent experiments each with two biological replicates. *P < 0.05 compared to NT. c Glucose uptake by tumoroids derived as in a normalized to protein concentration. Mean ± SEM. The data is from three independent experiments each with three biological replicates. *P < 0.05 compared to NT. #P < 0.05 compared to Polg KD. d Lactate levels in media from wells with tumoroids as in a normalized to DNA concentration. Mean ± SEM. The data is from three independent experiments each with two biological replicates, asterisk and number sign as in c. e Cell viability relative to untreated assayed by MTT in tumoroids derived as in a 72 h after mock treatment (Unt) or 0.5 mM H2O2. Mean ± SEM. The data is from three independent experiments each with two biological replicates. *P < 0.05 compared to NT

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